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ORIGINAL ARTICLE
Year : 2018  |  Volume : 5  |  Issue : 1  |  Page : 25-28

Exfoliative cytology for age estimation: A correlative study in different age groups


Department of Oral Pathology and Microbiology, Vivekanandha Dental College for Women, Tiruchengode, Tamil Nadu, India

Correspondence Address:
Thambu Keerthi Priyadharshini
Department of Oral Pathology and Microbiology, Vivekanandha Dental College for Women, Elaiyampalayam, Tiruchengode, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jiadsr.jiadsr_29_17

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Background: Oral exfoliative cytology is a simple and relatively pain-free procedure which can be carried out repeatedly with minimum discomfort to the patients. Oral exfoliative cytology has a sensitivity of 89.5%; its accuracy could be enhanced by cytomorphometric analysis of the cells. Aim and Objective: The present study was undertaken to evaluate the cell diameter (CD), nuclear diameter (ND), and nuclear-cytoplasmic ratio (N:C) and their variation with age using buccal smears. Materials and Methods: Buccal smears were collected from 100 apparently normal healthy individuals. The patients were divided into five groups 10–20, 21–30, 31–40, 41–50, and more than 50 years. The smears were fixed and stained. The CD and ND were measured using MS paint tool. The CD, ND, and nuclear-cytoplasmic (N:C) ratio were calculated for each case. Results: The cytomorphometric analysis of the exfoliated normal buccal mucosal cells revealed that there is a decrease in CD and ND with increasing age. No variation is found in N:C ratio. Conclusion: Age-related changes are observed in buccal smears, which could be helpful in age estimation.


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